poly-l-lysine, poly-l-arginine ... ...

gene vector


Transfectionreagent CHR (yellow solid):
Polycysteine-dendritic poly(His,Arg)1:5
209USD/2g   special promotion

It is composed of 100% amino acids, without any cytotoxic   PEI. CHR-mediated transfection does not require complex pre-formation, works well inserum-containing media and is biodegradable, which may prevent cumulative cytotoxicity and facilitates downstream processing.

CHR can be usedwith a broad range of celltypes. CHR could also be useful for in vivo genetransfer in gene therapy applications.



PEG—6 branched poly(his, epsilon-lys) (P6P, much safer siRNA vector for in vivo)

Cost-effective, scaled manufacturing

KB cell, LNCaP, PC-3, astrocytes, BSMC, HUVEC, NHEK



Dendriticpoly(stearyl-Lys, His, Arg) HCl (DSK)

Core: Dendriticpoly(Lys, Phe)

Subsurface:His-his-his

Surface: 5%Lys,5%stearyl-Lys, 90%Arg

162USD/mg (solid)

Features: highlyefficient, no observed toxicity, most inexpensive

Application:sensitive and primary cells

Description: DSKis a revolutionary transfection reagent that isn't made from cytotoxic PEI. Ithas been developed to cater for a wide range of cell types where othertransfection reagents have failed. Arginine residues play a critical role inintracellular uptake. Histidines have buffering capacity in the acidicenvironment of endosomes. Dendrimers with cationic and hydrophobic amino acidmotifs were reported to improve transfection by 6–10-fold over commercialreagents. DSK is suitable for delivery of plasmid DNA and siRNA.

Example (primary cells):

DSK binds pDNAnon-covalently, forminga nanoparticle. It is easy to use and has non-toxiceffects.

seed cells   ------------>   addDSK/pDNA complexes   ------------>   transfection assay

1. Plate cells oneday before the transfection experiment so that cells will be approximately 70%confluent onthe day of transfection.   
2. Combine 5mg DSK   with 5 ml PBS.
3. Combine 0.5 ug pDNA with 30 uL PBS.   
4. 30 uL DSK solution was added to this 30 uL pDNA-PBS gently and incubated for30 minutes at room temperature.   
5. DSK /pDNA complexes were added into each well while gently swirlingtheplate.   
6. Incubate cell at 37 C in a CO2 incubator. The transfection efficiencyofreporter gene could be analysis at 24-48 hours after adding the complexes.


Animal data willbe sent on request.



                                           

                                                    ICPNo. 11000739





                                   


Poly-L-Lysine
Email
huiyisu@126.com