Transfection reagent CHR (yellow solid): It is composed of 100% amino acids, without any cytotoxic PEI. CHR-mediated transfection does not require complex pre-formation, works well inserum-containing media and is biodegradable, which may prevent cumulative cytotoxicity and facilitates downstream processing. CHR can be usedwith a broad range of celltypes. CHR could also be useful for in vivo genetransfer in gene therapy applications.
PEG—6 branched poly(his, epsilon-lys) (P6P, much safer siRNA vector for in vivo) Cost-effective, scaled manufacturing KB cell, LNCaP, PC-3, astrocytes, BSMC, HUVEC, NHEK
Dendriticpoly(stearyl-Lys, His, Arg) HCl (DSK) Core: Dendriticpoly(Lys, Phe) Subsurface:His-his-his Surface: 5%Lys,5%stearyl-Lys, 90%Arg 162USD/mg (solid) Features: highlyefficient, no observed toxicity, most inexpensive Application:sensitive and primary cells Description: DSKis a revolutionary transfection reagent that isn't made from cytotoxic PEI. Ithas been developed to cater for a wide range of cell types where othertransfection reagents have failed. Arginine residues play a critical role inintracellular uptake. Histidines have buffering capacity in the acidicenvironment of endosomes. Dendrimers with cationic and hydrophobic amino acidmotifs were reported to improve transfection by 6–10-fold over commercialreagents. DSK is suitable for delivery of plasmid DNA and siRNA. Example (primary cells): DSK binds pDNAnon-covalently, forminga nanoparticle. It is easy to use and has non-toxiceffects. seed cells ------------> addDSK/pDNA complexes ------------> transfection assay 1. Plate cells oneday before the transfection experiment so that cells will be approximately 70%confluent onthe day of transfection.
Animal data willbe sent on request.
ICPNo. 11000739
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